Coding

Part:BBa_K3447003:Design

Designed by: Yingzhe Wei, Yushan Geng   Group: iGEM20_Jilin_China   (2020-09-05)


priR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 776
    Illegal XhoI site found at 505
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 349
    Illegal NgoMIV site found at 838
    Illegal NgoMIV site found at 1087
    Illegal NgoMIV site found at 1132
    Illegal NgoMIV site found at 1210
    Illegal NgoMIV site found at 1438
    Illegal AgeI site found at 868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 741


Design Notes

We added some nonsense mutation to avoid part rules.


Source

The gene is derived from Streptomyces pristinaespiralis, we found this sequence data in the reported EMBL/GenBank/DDBJ Nucleotide Sequence Database under the Accession Number X84072 by references below.

  • Conjugal Transferring of Resistance Gene ptr for Improvement of Pristinamycin-Producing Streptomyces pristinaespiralis. (2010), Appl. Biochem. Biotechnol., 160, 1853-1864.
  • Molecular characterization and transcriptional analysis of a multidrug resistance gene cloned from the pristinamycin-producing organism, Streptomyces pristinaespiralis. (1995), Molecular Microbiology, 17(5), 989-999.

References

1. Conjugal Transferring of Resistance Gene ptr for Improvement of Pristinamycin-Producing Streptomyces pristinaespiralis. (2010), Appl. Biochem. Biotechnol., 160, 1853-1864.

2. Molecular characterization and transcriptional analysis of a multidrug resistance gene cloned from the pristinamycin-producing organism, Streptomyces pristinaespiralis. (1995), Molecular Microbiology, 17(5), 989-999.